Human Cholecystokinin (CCK) ELISA Kit

Cat.#:

JL10747

Regulatory Status:

RUO

Alternative:

Cholecystokinin; CCK; Cleaved into: Cholecystokinin-58; CCK58; Cholecystokinin-58 desnonopeptide; (1-49)-CCK58; Cholecystokinin-39; CCK39; Cholecystokinin-33; CCK33; Cholecystokinin-25; CCK25; Cholecystokinin-18; CCK18; Cholecystokinin-12; CCK12; Cholecys

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

15.6-1000pg/mL

Sensitivity:

7.4pg/mL

Specificity:

This kit recognizes Human CCK in samples. No significant cross-reactivity or interference between Human CCK and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| CCK

Cholecystokinin (CCK or CCK-PZ) is a peptide hormone of the gastrointestinal system responsible for stimulating the digestion of fat and protein. Cholecystokinin, formerly called pancreozymin, is synthesized and secreted by enteroendocrine cells in the duodenum, the first segment of the small intestine. Its presence causes the release of pancreatic juice from the pancreas and bile from the gallbladder.CCK plays important physiological roles both as a neuropeptide in the central nervous system and as a peptide hormone in the gut.It is the most abundant neuropeptide in the central nervous system.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CCK spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Human CCK and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-105	87-108	105-115
1 : 4	Average (%)	94	98	110