| TF

Thymulin (also known as thymic factor or facteur thymique serique) is a nonapeptide produced by two distinct epithelial populations in the thymus.It requires zinc for biological activity. Its peptide sequence is H-Pyr-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn-OH.The hormone is believed to be involved in T-cell differentiation and enhancement of T and NK cell actions.Besides this rather paracrine or auto-organic effects on the thymus dependent immune system, thymulin seems to have neuroendocrine effects as well. There exist bidirectional interactions between thymic epithelium and the hypothalamus-pituitary axis (for example, thymulin follows a circadian rhythm and physiologically elevated ACTH levels correlate positively with thymulin plasma levels and vice versa).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	3000	1500	750	375	187.5	93.7	46.8	0
OD	0.188	0.27	0.389	0.642	0.929	1.38	1.766	1.842

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human TF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human TF and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
	Average (%)	93	98