Asymmetric Dimethylarginine (ADMA) ELISA Kit

Cat.#:

JL54657

Regulatory Status:

RUO

Alternative:

ADMA;2-Amino-5-[[amino(dimethylamino)methylidene]amino]pentanoic acid;N(G),N(G′)-Dimethylarginine

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.156-10μg/mL

Sensitivity:

0.069μg/mL

Specificity:

This kit recognizes ADMA in samples. No significant cross-reactivity or interference between ADMA and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| ADMA

Asymmetric dimethylarginine (ADMA) is a naturally occurring chemical found in blood plasma. It is a metabolic by-product of continual protein modification processes in the cytoplasm of all human cells. It is closely related to L-arginine, a conditionally essential amino acid. ADMA interferes with L-arginine in the production of nitric oxide (NO), a key chemical involved in normal endothelial function and, by extension, cardiovascular health.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (μg/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	0.216	0.298	0.417	0.669	0.955	1.405	1.79	1.865

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of ADMA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	97
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of ADMA and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-109	105-115
1 : 4	Average (%)	93	97	110