| Tetracycline

Tetracycline, sold under various brand names, is an antibiotic in the tetracyclines family of medications, used to treat a number of infections,including acne, cholera, brucellosis, plague, malaria, and syphilis.It works by inhibiting protein synthesis in bacteria.Tetracycline was originally made from bacteria of the genus Streptomyces.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.2	0
OD	0.216	0.298	0.417	0.669	0.955	1.405	1.79	1.865

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Tetracycline spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-103	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-107	105

 

| Linearity

Samples were spiked with high concentrations of Tetracycline and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-95	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	88-108	105-114
	Average (%)	94	98	108