| Tobramycin

Tobramycin is an aminoglycoside antibiotic derived from Streptomyces tenebrarius that is used to treat various types of bacterial infections, particularly Gram-negative infections. It is especially effective against species of Pseudomonas.Tobramycin does not pass the gastro-intestinal tract, so for systemic use it can only be given intravenously or by injection into a muscle.It was patented in 1965, and approved for medical use in 1974.It is on the World Health Organization's List of Essential Medicines.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	0.22	0.33	0.62	0.89	1.28	1.59	1.78	2.12

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Tobramycin spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	89-112	99
Plasma(n=8)	82-114	98
Cell Culture Supernates(n=8)	82-109	101

 

| Linearity

Samples were spiked with high concentrations of Tobramycin and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	85-115	100-110	86-99
	Average (%)	101	106	91
1 : 4	Range (%)	89-110	87-105	91-102
	Average (%)	103	97	98