Fish Cortisol (Cortisol) ELISA Kit

Cat.#:

JL18180

Regulatory Status:

RUO

Alternative:

Hydrocortisone

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.312-20ng/mL

Sensitivity:

0.158ng/mL

Specificity:

This kit recognizes Fish Cortisol in samples. No significant cross-reactivity or interference between Fish Cortisol and analogues was observed.

Sample Type:

Serum;plasma;urine and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| Cortisol

Cortisol is a steroid hormone in the glucocorticoid class of hormones and a stress hormone. When used as medication, it is known as hydrocortisone.It is produced in many animals, mainly by the zona fasciculata of the adrenal cortex in an adrenal gland.In other tissues, it is produced in lower quantities.By a diurnal cycle, cortisol is released and increases in response to stress and a low blood-glucose concentration.It functions to increase blood sugar through gluconeogenesis, suppress the immune system, and aid in the metabolism of calories.It also decreases bone formation.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Fish Cortisol spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-104	101

| Linearity

Samples were spiked with high concentrations of Fish Cortisol and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
1 : 2	Average (%)	92	93
1 : 4	Range (%)	89-104	87-107
1 : 4	Average (%)	93	98