ELISA, enzyme-Linked Immunosorbent Assay, is a kind of high sensitive experiment technology that is based on the immunological reaction and combines the specific reactions of antigens and antibodies with the efficient catalysis of enzymes to substrate. This method not only can be used to measure antibodies, but also can be used to determine antigens in samples, so it has been widely used in clinical testing and research field.

1.Double Antibody Sandwich is used to test the antigens
2.The indirect method is used to test the antibodies
3.Provide various ELISA technology to sample testing

1.Samples to be tested(serum, plasma or cell culture supernatants).
2.Antigens (for antigen identification) are required to be provided by customer, and the purity and quality of the antigen will be determined according to the experimental requirements.
3.Antibodies (for antibody identification) are required to be provided by customer or EIAab. Meanwhile, please provide the detailed instructions of commercial antibodies, so that we can follow the instructions accurately.
4.Other information including: Specimen number; The project; Whether to make the complex hole; Whether the specimen is mailed back after the experiment.

Must be clear whether the detection composition in sample is stable before collecting samples. Fresh samples are first choice, if not, avoid freeze-thaw of samples. Samples to be used within 5 days may be stored at 2-8℃, otherwise samples must be stored at -20℃ (1 month) or -80℃ (2 months) to avoid loss of bioactivity and contamination. Samples must be liquid contains no precipitation, including serum, plasma, cell culture supernatants, tissue homogenate, urine and other biological samples. Besides, the volume of sample should be better more than 500μL.
1.Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 20 minutes at approximately 1000×g, remove serum and assay immediately. If precipitation occurs, should be centrifuged again.
2.Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2℃-8℃ within 30 minutes of collection. If precipitation occurs, should be centrifuged again.
3.Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, after cutting samples and then weighing the samples. tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20mL of 1XPBS and stored overnight at ≤ -20℃ After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000xg. Remove the supernatant and assay immediately. If precipitation occurs, should be centrifuged again.
4.Cell culture supernatants - Collected secretory ingredients with a sterile tube, centrifuge samples for 20 minutes at 1000×g, collected the supernatant. Detection of intracellular components, diluted with PBS cell suspension, making the cell concentration reach up to about 1 million/mL. By repeated freezing and thawing, so that the cell membrane damage and release intracellular components, centrifuge samples for 20 minutes at 1000×g, collect the supernatant. Preservation process if the formation of precipitation should be centrifuged again.
5.Urine and other biological samples - Collected with a sterile tube, centrifuge samples for 20 minutes at 1000×g, carefully collected the supernatant. If precipitation occurs, should be centrifuged again.

The experimental report

1.From the date of receipt of the sample, 5-10 working days complete the test, and provide test report.
2.Due to the particularity of biological samples, we guarantee that the experimental results are objective and reliable, but do not bear any responsibility for the failure of testing as the reasons of the experimental samples themselves (such as protein degradation, sample contamination, transportation loss, etc.).
3.Scientific research is challenging and exploratory, so we cannot absolutely guarantee the experiment successful, nor does it guarantee the results are in line with customer expectations, however, we will do the best to ensure that the experiment itself is going well. We also hope that you will provide us with sample information in as much detail as possible without affecting the confidentiality of the experiment. We guarantee that the relevant data related to the experiment does not disclose to you and third parties and other than your designated contact.
4.The test will consume samples, if the test results do not meet the expectations, we will not pay for the sample.

ELISA, enzyme-Linked Immunosorbent Assay, is a kind of high sensitive experiment technology that is based on the immunological reaction and combines the specific reactions of antigens and antibodies with the efficient catalysis of enzymes to substrate. This method not only can be used to measure antibodies, but also can be used to determine antigens in samples, so it has been widely used in clinical testing and research field.

1.Double Antibody Sandwich is used to test the antigens
2.The indirect method is used to test the antibodies
3.Provide various ELISA technology to sample testing

1.Samples to be tested(serum, plasma or cell culture supernatants).
2.Antigens (for antigen identification) are required to be provided by customer, and the purity and quality of the antigen will be determined according to the experimental requirements.
3.Antibodies (for antibody identification) are required to be provided by customer or EIAab. Meanwhile, please provide the detailed instructions of commercial antibodies, so that we can follow the instructions accurately.
4.Other information including: Specimen number; The project; Whether to make the complex hole; Whether the specimen is mailed back after the experiment.

Must be clear whether the detection composition in sample is stable before collecting samples. Fresh samples are first choice, if not, avoid freeze-thaw of samples. Samples to be used within 5 days may be stored at 2-8℃, otherwise samples must be stored at -20℃ (1 month) or -80℃ (2 months) to avoid loss of bioactivity and contamination. Samples must be liquid contains no precipitation, including serum, plasma, cell culture supernatants, tissue homogenate, urine and other biological samples. Besides, the volume of sample should be better more than 500μL.
1.Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 20 minutes at approximately 1000×g, remove serum and assay immediately. If precipitation occurs, should be centrifuged again.
2.Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2℃-8℃ within 30 minutes of collection. If precipitation occurs, should be centrifuged again.
3.Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, after cutting samples and then weighing the samples. tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20mL of 1XPBS and stored overnight at ≤ -20℃ After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000xg. Remove the supernatant and assay immediately. If precipitation occurs, should be centrifuged again.
4.Cell culture supernatants - Collected secretory ingredients with a sterile tube, centrifuge samples for 20 minutes at 1000×g, collected the supernatant. Detection of intracellular components, diluted with PBS cell suspension, making the cell concentration reach up to about 1 million/mL. By repeated freezing and thawing, so that the cell membrane damage and release intracellular components, centrifuge samples for 20 minutes at 1000×g, collect the supernatant. Preservation process if the formation of precipitation should be centrifuged again.
5.Urine and other biological samples - Collected with a sterile tube, centrifuge samples for 20 minutes at 1000×g, carefully collected the supernatant. If precipitation occurs, should be centrifuged again.

The experimental report

1.From the date of receipt of the sample, 5-10 working days complete the test, and provide test report.
2.Due to the particularity of biological samples, we guarantee that the experimental results are objective and reliable, but do not bear any responsibility for the failure of testing as the reasons of the experimental samples themselves (such as protein degradation, sample contamination, transportation loss, etc.).
3.Scientific research is challenging and exploratory, so we cannot absolutely guarantee the experiment successful, nor does it guarantee the results are in line with customer expectations, however, we will do the best to ensure that the experiment itself is going well. We also hope that you will provide us with sample information in as much detail as possible without affecting the confidentiality of the experiment. We guarantee that the relevant data related to the experiment does not disclose to you and third parties and other than your designated contact.
4.The test will consume samples, if the test results do not meet the expectations, we will not pay for the sample.

ELISA, enzyme-Linked Immunosorbent Assay, is a kind of high sensitive experiment technology that is based on the immunological reaction and combines the specific reactions of antigens and antibodies with the efficient catalysis of enzymes to substrate. This method not only can be used to measure antibodies, but also can be used to determine antigens in samples, so it has been widely used in clinical testing and research field.

1.Double Antibody Sandwich is used to test the antigens
2.The indirect method is used to test the antibodies
3.Provide various ELISA technology to sample testing

1.Samples to be tested(serum, plasma or cell culture supernatants).
2.Antigens (for antigen identification) are required to be provided by customer, and the purity and quality of the antigen will be determined according to the experimental requirements.
3.Antibodies (for antibody identification) are required to be provided by customer or EIAab. Meanwhile, please provide the detailed instructions of commercial antibodies, so that we can follow the instructions accurately.
4.Other information including: Specimen number; The project; Whether to make the complex hole; Whether the specimen is mailed back after the experiment.

Must be clear whether the detection composition in sample is stable before collecting samples. Fresh samples are first choice, if not, avoid freeze-thaw of samples. Samples to be used within 5 days may be stored at 2-8℃, otherwise samples must be stored at -20℃ (1 month) or -80℃ (2 months) to avoid loss of bioactivity and contamination. Samples must be liquid contains no precipitation, including serum, plasma, cell culture supernatants, tissue homogenate, urine and other biological samples. Besides, the volume of sample should be better more than 500μL.
1.Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 20 minutes at approximately 1000×g, remove serum and assay immediately. If precipitation occurs, should be centrifuged again.
2.Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2℃-8℃ within 30 minutes of collection. If precipitation occurs, should be centrifuged again.
3.Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, after cutting samples and then weighing the samples. tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20mL of 1XPBS and stored overnight at ≤ -20℃ After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000xg. Remove the supernatant and assay immediately. If precipitation occurs, should be centrifuged again.
4.Cell culture supernatants - Collected secretory ingredients with a sterile tube, centrifuge samples for 20 minutes at 1000×g, collected the supernatant. Detection of intracellular components, diluted with PBS cell suspension, making the cell concentration reach up to about 1 million/mL. By repeated freezing and thawing, so that the cell membrane damage and release intracellular components, centrifuge samples for 20 minutes at 1000×g, collect the supernatant. Preservation process if the formation of precipitation should be centrifuged again.
5.Urine and other biological samples - Collected with a sterile tube, centrifuge samples for 20 minutes at 1000×g, carefully collected the supernatant. If precipitation occurs, should be centrifuged again.

The experimental report

1.From the date of receipt of the sample, 5-10 working days complete the test, and provide test report.
2.Due to the particularity of biological samples, we guarantee that the experimental results are objective and reliable, but do not bear any responsibility for the failure of testing as the reasons of the experimental samples themselves (such as protein degradation, sample contamination, transportation loss, etc.).
3.Scientific research is challenging and exploratory, so we cannot absolutely guarantee the experiment successful, nor does it guarantee the results are in line with customer expectations, however, we will do the best to ensure that the experiment itself is going well. We also hope that you will provide us with sample information in as much detail as possible without affecting the confidentiality of the experiment. We guarantee that the relevant data related to the experiment does not disclose to you and third parties and other than your designated contact.
4.The test will consume samples, if the test results do not meet the expectations, we will not pay for the sample.

ELISA, enzyme-Linked Immunosorbent Assay, is a kind of high sensitive experiment technology that is based on the immunological reaction and combines the specific reactions of antigens and antibodies with the efficient catalysis of enzymes to substrate. This method not only can be used to measure antibodies, but also can be used to determine antigens in samples, so it has been widely used in clinical testing and research field.

1.Double Antibody Sandwich is used to test the antigens
2.The indirect method is used to test the antibodies
3.Provide various ELISA technology to sample testing

1.Samples to be tested(serum, plasma or cell culture supernatants).
2.Antigens (for antigen identification) are required to be provided by customer, and the purity and quality of the antigen will be determined according to the experimental requirements.
3.Antibodies (for antibody identification) are required to be provided by customer or EIAab. Meanwhile, please provide the detailed instructions of commercial antibodies, so that we can follow the instructions accurately.
4.Other information including: Specimen number; The project; Whether to make the complex hole; Whether the specimen is mailed back after the experiment.

Must be clear whether the detection composition in sample is stable before collecting samples. Fresh samples are first choice, if not, avoid freeze-thaw of samples. Samples to be used within 5 days may be stored at 2-8℃, otherwise samples must be stored at -20℃ (1 month) or -80℃ (2 months) to avoid loss of bioactivity and contamination. Samples must be liquid contains no precipitation, including serum, plasma, cell culture supernatants, tissue homogenate, urine and other biological samples. Besides, the volume of sample should be better more than 500μL.
1.Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 20 minutes at approximately 1000×g, remove serum and assay immediately. If precipitation occurs, should be centrifuged again.
2.Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2℃-8℃ within 30 minutes of collection. If precipitation occurs, should be centrifuged again.
3.Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, after cutting samples and then weighing the samples. tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20mL of 1XPBS and stored overnight at ≤ -20℃ After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000xg. Remove the supernatant and assay immediately. If precipitation occurs, should be centrifuged again.
4.Cell culture supernatants - Collected secretory ingredients with a sterile tube, centrifuge samples for 20 minutes at 1000×g, collected the supernatant. Detection of intracellular components, diluted with PBS cell suspension, making the cell concentration reach up to about 1 million/mL. By repeated freezing and thawing, so that the cell membrane damage and release intracellular components, centrifuge samples for 20 minutes at 1000×g, collect the supernatant. Preservation process if the formation of precipitation should be centrifuged again.
5.Urine and other biological samples - Collected with a sterile tube, centrifuge samples for 20 minutes at 1000×g, carefully collected the supernatant. If precipitation occurs, should be centrifuged again.

The experimental report

1.From the date of receipt of the sample, 5-10 working days complete the test, and provide test report.
2.Due to the particularity of biological samples, we guarantee that the experimental results are objective and reliable, but do not bear any responsibility for the failure of testing as the reasons of the experimental samples themselves (such as protein degradation, sample contamination, transportation loss, etc.).
3.Scientific research is challenging and exploratory, so we cannot absolutely guarantee the experiment successful, nor does it guarantee the results are in line with customer expectations, however, we will do the best to ensure that the experiment itself is going well. We also hope that you will provide us with sample information in as much detail as possible without affecting the confidentiality of the experiment. We guarantee that the relevant data related to the experiment does not disclose to you and third parties and other than your designated contact.
4.The test will consume samples, if the test results do not meet the expectations, we will not pay for the sample.